There was also a significant increase in the frequency of NK cells and Ki67+ NK cells in the TME; interestingly, the levels of splenic CD69+ and Ki67+ NK cells were also elevated, potentially suggesting mobilization of NKs from the spleen to the tumor (Fig. S17).46 Trends towards increased frequency of MDSCs (Fig. 3d,e), a significant increase in the frequency of FoxP3+CD4+ regulatory T cells (Fig. 3e,f), and elevated MHC-II and PD-L1 on macrophages (Fig. 3g,h) was also observed. This evidence concerns the gene CD4 and neoplasm.