To further explore the functional effect of PKC modulators on NK cells and compare them with the effects of SUW133, we cultured NK cells for 24 hours in either bryostatin-1, prostratin, or SUW133 in parallel with untreated cells, then incubated in a 4-hour lysis assay with K562 cells, a human leukemia cell line that lacks HLA-antigen expression [100], at various effector-to-target (E:T) ratios (Figure 5). This evidence concerns the gene PRRT2 and leukemia.