In this study, we selected the PRV gE protein as our research target for the following reasons: first, the gE protein is a glycoprotein located in the viral envelope, which is commonly used to detect wild-type virus infection, and its antibody detection can also distinguish between wild-type virus infection and vaccine immunization animals (Nauwynck, 1997; Ao et al., 2003). Here, ART4 is linked to viral infectious disease.