CEACAM5 and neoplasm: The development of various fluorescent probes and instruments also contributes to the continuing evolution of FIA.2,71,72 Multiple FIA-related technologies with high detection sensitivities and various measurable properties have been developed, including fluorescent excitation transfer immunoassay, fluorescence polarization immunoassay, and time-resolved fluorescence immunoassays.73–77 For example, the multicolor quantum dots based on fluorescence polarization immunoassay have been applied in the detection of tumor biomarkers such as α-AFP and CEA.78