CRISPR/Cas9 system is extensively used for different kinds of cancer biomarkers including virus nucleic acids, ctDNAs (i.e., EGFR mutation), miRNAs (i.e., miR-17, miR-31), proteins (i.e., TGF-β1, CEA, PSA, AFP), and extracellular vesicles.90 CRISPR/Cas9 can combine with other assays for tumor biomarker identification, such as qPCR, FISH, and nanotechnology, providing an efficient way for tumor biomarker discovery. This evidence concerns the gene EGFR and neoplasm.