To further investigate the DEPDC5 function in T cells in vivo, MC38 colon carcinoma cells (5 × 105 cells per mouse) were injected subcutaneously into 6-week-old Depdc5ncl and Depdc5tko mice, and tumor volumes were measured on alternate days (starting from day 10 after inoculation). This evidence concerns the gene DEPDC5 and neoplasm.