In external queue validation, the results for S100A16 (IVW, OR = 0.99, 95% CI, 0.91–1.07, p = 0.722), S100A14 (Wald ratio, OR = 1.08, 95% CI, 0.90–1.30, p = 0.406) and PDE5A (Wald ratio, OR = 0.99, 95% CI, 0.98–1.00, p = 0.105) were not replicated, while the results for MIA (IVW, OR = 0.90, 95% CI, 0.83–0.97, p = 0.009) were replicated in non-small cell lung cancer (Fig. 3). This evidence concerns the gene S100A16 and non-small cell lung carcinoma.