In addition, the effect of IL-1β on autophagy in ESCC cells was assessed by immunofluorescence assay to detect the aggregation degree of LC3B and p62, and it was found that the aggregation degree of LC3B was enhanced and that of p62 was weakened in EC109 cells knocked down with IL-1β, and that LC3B aggregation degree was weakened and p62 aggregation degree was enhanced in KYSE150 cells overexpressing IL-1β. Here, IL1B is linked to esophageal squamous cell carcinoma.