Therefore, we used lentivirus and plasmid to transfect CRC cell lines and divided the cells into the control group (Vector), PD-L1 overexpression group (PD-L1), PD-L1 overexpression+sh-NC group (PD-L1+sh-NC) and PD-L1 overexpression + IPO5 knockdown group (PD-L1 + sh-IPO5) (Fig. 8G). This evidence concerns the gene CD274 and colorectal carcinoma.