To investigate the significance of AMPK in all bone marrow-derived and tissue-resident myeloid cells, we used male and female Flox and MacKO mice, and induced NASH over the course of 8 weeks using a CDAHFD, which is known to produce hepatic steatosis, inflammation, and F2 fibrosis (37). The gene discussed is PRKAA1; the disease is metabolic dysfunction-associated steatohepatitis.