The MAVS signaling pathway stimulates the expression of proinflammatory cytokines,22,23 which are implicated in the development of MASLD.24 We evaluated inflammatory markers by measuring hepatic TNFα, IL-1β, IKKβ, and NFκβ expression in control or MAVS knockdown mice fed an MCD diet. The gene discussed is TNF; the disease is metabolic dysfunction-associated steatotic liver disease.