Our findings revealed hepatic inhibition of PI3K/Akt pathway and reduction in bcl2 levels and the elevation in hepatic cleaved caspase 3, p53, and bax levels during the intrahepatic cholestasis induced by ANIT, which further suggest the activation of oxidative stress condition and induction of the apoptotic pathways. Here, CASP3 is linked to intrahepatic cholestasis.