Next-generation sequencing (NGS) of 201 genes showed a higher frequency of PIK3CA (0 out of 65 (0.0%) versus 9 out of 60 (15.0%)) and TP53 (6 out of 65 (9.23%) versus 19 out of 60 (31.67%)) mutations in high-neural tumors (Extended Data Fig. 5b,c). This evidence concerns the gene PIK3CA and nervous system cancer.