To further analyse the induction of PRMT5 expression by STAT3 in NSCLC cells, we generated STAT3-knockout (STAT3-KO) cells by using the CRISPR/Cas9 system in A549 (EGFR wild type, adenocarcinoma) and HCC827 cells (EGFR mutant, adenocarcinoma). The gene discussed is STAT3; the disease is adenocarcinoma.