Furthermore, we discovered that NEK2 overexpression together with markers of T-cell exhaustion, such as PD-1, LAG3, CTLA4 and TIGIT, indicated the worst prognosis in ccRCC (Fig. 6D, Supplementary Figure 7A-C, http://links.lww.com/MD/M261). This evidence concerns the gene LAG3 and nonpapillary renal cell carcinoma.