HRAS and neoplasm: When compared with the 3 RAS gene isoforms across all tumor subtypes, the profiles of interpatient variabilities were delineated at the VAF levels ranging from 0.15% to 51.53%, specifically from 0.59% to 51.53% for NRAS, from 0.36% to 43.56% for HRAS, and from 0.15% to 46.64% for KRAS variants, with no significant difference among 3 isoforms (P = .16) (Figure 1; eFigure 2 in Supplement 1).