HMGA1 and neoplasm: At 72 h after treating our 3D cultures with 10 nM trabectedin, HMGA1 knockdown affected the capacity of tumor spheroids to grow: shHMGA1 spheroids showed a significant decrease in their compacted area, compared to the shControl (Fig. 3F): 0.230 mm2 ± 0.067mm2 (shControl) vs. 0.083 mm2 ± 0.020 mm2 (shHMGA1-281), p < 0.001 and 0.230 mm2 ± 0.067 mm2 (shControl) vs. 0.029 mm2 ± 0.033 mm2 (shHMGA1-446), p < 0.001 (Fig. 3G).