However, after hydrogen therapy by Mg‐CaCO3, both ifn‐γ and tnf‐α in mice serum distinctly increased in the 4T1 cell model (ifn‐γ: Ctrl: 7.736, Mg‐OPSZ: 25.92, Mg‐CaCO3: 34.56; tnf‐α: Ctrl: 6.780, Mg‐OPSZ: 3.320, Mg‐CaCO3: 51.91) (Figure 6q,r) and the MC38 cell model (ifn‐γ: Ctrl: 20.96, Mg‐OPSZ: 17.34, Mg‐CaCO3: 42.54; tnf‐α: Ctrl: 17.73, Mg‐OPSZ: 23.95, Mg‐CaCO3: 54.69) (Figure S28, Supporting Information), confirming the activation of systematic anti‐tumor immunity. This evidence concerns the gene IFNG and neoplasm.