ANO6 and gastrointestinal stromal tumor: Furthermore, RT-qPCR and western blotting assays revealed that the ANO6-plasmid increased Bax expression (Figure 4f and g) and reduced Bcl-2 expression (Figure 4f and h) in GIST-T1 IR cells, indicating that ANO6 (TMEM16F) is a vital regulator of GIST progression.