Moreover, we detected ferroptosis-related gene expression in ANO6-plasmid transfected GIST-T1 IR cells, and our data demonstrated that the upregulation of ANO6 inhibited Cys, GSH, and GPX4 levels (Figure 7f–h), as well as SLC7A11 and SLC3A2 expression (Figure 7i–k), compared to the control-plasmid group. This evidence concerns the gene GPX4 and gastrointestinal stromal tumor.