Immunohistochemistry assays suggested that the ANO6-plasmid prominently increased ANO6 (TMEM16F) expression and reduced SLC7A11 and SLC3A2 expression, demonstrating that ANO6 (TMEM16F) inhibited GIST growth by regulating SLC7A11 and SLC3A2. This evidence concerns the gene SLC3A2 and gastrointestinal stromal tumor.