However, the YB-1 has no effect on the expression level of miR-223 in the cells in anti-inflammatory microglia and AD cell model (Fig. 5C and S1F) and also PTEN level in AD cell model (Figure S1G), which further demonstrate that YB-1 is involved in the packaging of miR-223 into EXO rather than regulating regeneration or expression. This evidence concerns the gene YBX1 and Alzheimer disease.