Moreover, 5-FU can excite the autoimmune system through the 5-FU induced-immunogenic cell death (ICD) effect, such as calreticulin (CRT) eversion, high mobility group protein 1 (HMGB1) efflux, etc., activating dendritic cells (DCs) and increase T cells infiltration, as well as activation tumor local immunity [21]. The gene discussed is CALR; the disease is neoplasm.