BCL2 and melanoma: In order to deeper characterize the specific functions of Bcl-2, we performed RNA-seq analysis after siRNA-mediated transient knockdown of either Bcl-2 (si-Bcl-2) or Bcl-xL (si-Bcl-xL) in human melanoma A375 cell line (protein and mRNA knockdown validation in Supplementary Figure S1).