To clarify the role of the cGAS-STING-NF-κB/IFNβ signaling pathway in the anti-tumor response to zebularine, we treated Cgas-/-, Sting-/-, and sh-Rela/p65 B16F10 tumors implanted into wild-type mice, and sh-Ifnar1 B16F10 tumors implanted into IFNAR1-/- mice. The gene discussed is CGAS; the disease is neoplasm.