To analyze the protein expression of ASRGL1 in ALS and controls, we stained formalin-fixed paraffin embedded (FFPE) brain cortical samples (Broadman area 6 (BA6); pre-motor cortex) from ALS (n = 4), Alzheimer’s disease (n = 4), Multiple Sclerosis (n = 4), and non-neurological controls (n = 4) with an ASRGL1 antibody, using an automated staining system, to avoid differences due to manual staining. Here, ASRGL1 is linked to early-onset autosomal dominant Alzheimer disease.