PGLYRP1 and colorectal cancer: Additionally, we explored a more general link between PGLYRP1 expression and CSC features by assessing PGLYRP1 expression in different cancer cell lines and observed an enrichment in PGLYRP1 expression in spheroids in all cell lines tested, except for colorectal cancer cells, suggesting that PGLYRP1 could also be important in CSCs of other tumour types, but it is not a pan-CSC marker (online supplemental figure S8D).