After validation of the MKRN2 siRNA pool by Western blotting (Fig 4A), we performed multicycle infections of A549 MKRN2 depleted cells with A/WSN/33, the 2009 pandemic H1N1 A/California/7/2009 virus and an H3N2 A/Norway/466/2014 strain. Here, MKRN2 is linked to infection.