The expression of the 7 signature genes across the 8 clusters of cells, namely Egr1, Tfrc, Jun, Cxcl2, Ctsb, l1b, and Gpx4, were validated in the kidneys of IRI mice and controls, and revealed that Egr1, Jun and Cxcl2 expression was significantly higher in AKI mice (Fig. 6c). The gene discussed is GPX4; the disease is acute kidney injury.