Multiplex immunofluorescence staining on renal pathology biopsy samples from human AKI patients further confirmed the presence of Egr1 + PTC-C1, Jun + PTC-C3, Cxcl2 + Mac-C1, and Egr1 + Fib-C1 cells during the occurrence of AKI. This evidence concerns the gene C3 and acute kidney injury.