To reveal the mechanism of a congenital heart disease and arrhythmia linked Cx45 variant R184G [45], we expressed this variant in DKO HEK293 cells in two expression vectors, i.e. GFP untagged Cx45 R184G-IRES-GFP and GFP tagged Cx45 R814G-GFP for functional and morphological studies respectively. This evidence concerns the gene GJC1 and congenital heart disease.