After referring to many literatures and based on the previous research of our research group,10, 24, 25, 26, 27, 28 we treated MRC5 cells with 5 ng/mL TGF‐β1 for 48 h to obtain tumor‐associated fibroblasts (CAFs), which were later referred to as CAFs. The gene discussed is TGFB1; the disease is neoplasm.