Analysis the differences of proteins with or without PPIs, we observed that the phosphorylated levels of GSK3β‐Ser9, EGFR‐Y1086, ERK‐Thr202/204, and CREB‐Ser133 were upregulated, while the P53 and PYK2‐Y402 was decreased in human H441 tumor cells(Figure 4B,C). This evidence concerns the gene CREB1 and neoplasm.