46 Pereira et al. discovered that GLUT1 expression in MCF7 breast cancer spheroids was 0.5-fold more significant than in monolayer cultures. 47 Furthermore, Khaitan et al. studied increased glucose consumption and lactate production two‐ to three-fold higher in BMG‐1 glioma spheroids than in BMG‐1 monolayer cell cultures. 44 It has been observed that increased GLUT1 and lactate generation due to the high glycolytic rate of cancer cells can contribute to drug resistance via altered expression of the multidrug resistance-associated protein 1 and P-glycoprotein. 48. This evidence concerns the gene SLC2A1 and central nervous system cancer.