Madsen et al. developed multi-cellular tumor spheroids consisting of cancer cells, fibroblasts, and macrophages to study the inhibition and reprogramming of TAM initiated by TAM inhibiting compounds, that is, CCL2 Ab, CSF1R inhibitor, CSF1R Ab, poly I: C, CD40 Ab, and CD40 ligand for their polarization. The gene discussed is CCL2; the disease is neoplasm.