Our results indicate that tumor infiltrating lymphocytes have significance in patient outcome also in cholangiocarcinoma as Immune cell score, based on CD3+ and CD8+ lymphocyte densities at tumor center and invasive margin, was a strong independent prognostic factor for overall survival when adjusted with age, sex, TNM stage, radicality of surgery, tumor location, and PD-L1IC expression. This evidence concerns the gene CD8A and neoplasm.