Gao and colleagues established in 2014 for the first time the long term fully characterized cultured PC organoid platform derived from advanced and metastatic PC tissues, which recapitulates molecular diversity of PC and showed TMPRSS2-ERG fusion, SPOP mutation, SPINK1 overexpression, and CHD1 loss as well as mutations in DNA repair pathway. This evidence concerns the gene SPOP and pachyonychia congenita.