To determine if PAX6 modulates CXCL14, we developed a PAX6-knockdown HCE-2 cell line via lentiviral infection and confirmed via Western blot that CXCL14 expression markedly decreased with PAX6 suppression, as seen in Fig. 6C. RNA sequencing of these PAX6-downregulated cells revealed significant suppression of CXCL14, as seen in Fig. 6B, D, indicating PAX6’s regulatory role on CXCL14 expression. This evidence concerns the gene CXCL14 and infection.