RIPK3 and systemic inflammatory response syndrome: To demonstrate scalability, we used automated immunohistochemistry to characterize the expression of Caspase-8, RIPK1 and RIPK3 across six tissues during TNF-induced systemic inflammatory response syndrome (SIRS)—a widely-used model of RIPK-dependent pathology (Fig. 3A; (Duprez et al, 2011; Harris et al, 2017; Newton et al, 2016; Newton et al, 2014; Zelic et al, 2018)).