PKM2–PGAM1 interaction was further confirmed in a panel of highly proliferating tumor cells, including A549, H1299, MCF-7, MDA-MB-231, AGS, and Jurkat, but hardly detectable in untransformed cells, including HBE-2, MCF-10A, GES-1, activated T and naive T cells (Figs. 1F and EV1A–C), suggesting a pan-cancer existence of the interaction between these two metabolic enzymes. This evidence concerns the gene PKM and neoplasm.