To evaluate whether the integrin α2β1–CXCL1 and lytC_22–Slamf4 axes function independently of each other, we determined the effect of anti-Slamf4 on P. anaerobius-induced CXCL1 by CRC in vitro as well as in MC38 allografts. The gene discussed is CD244; the disease is colorectal carcinoma.