Consequently, the purpose of this study is to quantify the levels of CD69, CD71, CD40L an HLADR- bearing CD3 (pan T), CD4 and CD8 cells, and CB1, CD152 and GARP-bearing CD25+ FoxP3+ Treg cells in the acute phase of MDD, and to determine if activation of T cells and depletion of Treg are associated with the severity of the acute phase. The gene discussed is CTLA4; the disease is major depressive disorder.