To understand how the proteostasis regulation fits in the in vivo highly spontaneous BRAFV600E-induced tumorigenesis where Tbx3 is re-activated and critically required for tumor initiation and progression15, we first collected tumor tissues from mPTC model generated by crossing thyroid peroxidase TPO-Cre with LSL-BrafV600E(Supplementary Fig. 2h)43. Here, TPO is linked to neoplasm.