Cell lines with different molecular backgrounds were included here, such as mouse embryonic fibroblasts (MEFs), BRAFV600E-mutated papillary thyroid cancer (PTC) K1 cells and anaplastic thyroid cancer (ATC) 8505 C cells, as well as an estrogen receptor positive (ER + ) breast cancer cell line MCF-7, and KRAS-mutated lung adenocarcinoma cell line A549, and showed different turnover rates. The gene discussed is ESR1; the disease is breast carcinoma.