To examine the impact of glucose consumption on cell survival, SPINK4-overexpressing CRC cells were treated with low glucose (0.5 mM Glu), a glucose transport inhibitor (Cyto-B, 20 μM/L), and a hexokinase-2 inhibitor (3-BrPA, 10 μg/mL) for 48 h. This evidence concerns the gene HK2 and colorectal carcinoma.