We used HEK293T (embryonic kidney cells), HeLa S3 (cervix cancer cells), U2OS (osteosarcoma cells), MIA PaCa-2 (pancreatic cancer cells), PDAC087T (patient-derived pancreatic cancer cells), and Jurkat cells (T cell acute lymphoblastic leukemia cells) that stably expressed the H2B-GFP-ALFA-KRASG12V166 construct (Figure S3A). Here, H2BC21 is linked to osteosarcoma.