Inhibited tumor growth, suppressed cell proliferation, blocked cell cycle was in the G0/G1 phase, reduced the number of cancer stem cells, reversed hepatocellular carcinoma malignant phenotype, downregulated the activity of the AKT/GSK-3β/β-catenin axis, inhibits epithelial to mesenchymal transition induced by TGF-β. This evidence concerns the gene GSK3B and hepatocellular carcinoma.