Differential analysis of the above single-cell data showed high frequency occurrence of Clec4e, Retnlg and S100a9 as genes unique to the top50 DEGs in the pulmonary group, whereas Coro1a and Lars2, were genes that were highly frequent and unique to the top50 DEGs in the extra-pulmonary ALI. The gene discussed is S100A9; the disease is acute respiratory distress syndrome.