In conclusion, our study demonstrated that H2 inhalation significantly rescued LPS-induced BPD, and the alleviation of LPS-induced BPD by H2 inhalation was mainly dependent on the inhibition of the TLR4–NFκB–IL6/NLRP3-mediated signaling pathway, eventually resulting in scavenging inflammatory cytokines/chemokines. This evidence concerns the gene TLR4 and bronchopulmonary dysplasia.