PAX6 and neoplasm: Interestingly, other than these regions with increased chromatin accessibility, we also observed several decreased regions of chromatin accessibility after overexpression of PAX6. The top 2 significantly decreased regions of chromatin accessibility were “negative regulation of peptidyl-serine phosphorylation of STAT protein (p value < 0.03)” and “histone H4-K20 trimethylation (H4K20me3) (p value < 0.03)”, which indicated an inhibition on the process of negative regulation of STAT signaling and an attenuation of the tumor suppressive H4K20me3 after PAX6 overexpression (Fig. 8c).