PAX6 and neoplasm: Finally, our sequencing results also show the enrichment of signaling pathways related to axons guidance and nerve filament development and assembly in PCa cells with a high expression of PAX6. The last neuronal features might be related to additional potential function in tumor metastasis or possible interactions with nerve cells or other cells such as immune cells [64, 65] in the tumor microenvironment to enhance the aggressiveness and therapy resistance, which is a subject of future studies.