First, by bioinformatics and experimental assays, we revealed a positive correlation between the expression of PAX6 and the expression of STAT5A or MET. Second, overexpression of STAT5A upregulates the expression of NE signature genes such as SYP, CHGA, ENO2 and NCAM1. Third, by rescue assays in vitro and in vivo, knockdown of STAT5A reverses the phenotype of NE trans-differentiation in PCa, even under the condition of PAX6 overexpression. Here, ENO2 is linked to posterior cortical atrophy.