To ascertain the competition between PVT1 and PLAG1 in the binding of miR-195-5p within HCC cells, the anti-AGO2 RIP assay was employed to demonstrate AGO2's efficient capture by anti-AGO2 antibodies in MHCC97H cells transfected with miR-195-5p mimics (Fig. 4K), and suggested competition between PVT1 and PLAG1 for miR-195-5p binding within HCC cells. The gene discussed is AGO2; the disease is hepatocellular carcinoma.