Studies have shown that not all hepatocellular carcinomas secrete high levels of AFP, and that high levels of AFP can be detected in the serum of some patients with cirrhosis and chronic hepatitis35, so in this paper, we set up an AFP-negative HCC group for differential diagnosis, and we found that the combined index of ApoB/ApoA1 ratio and AFP has a good diagnostic value. The gene discussed is AFP; the disease is hepatocellular carcinoma.