Among these genes, some were related to RNA processing such as SF3B4, other genes encoded ribosomal proteins such as MRPL54, or served as transcription factors such as ZNF207. To further evaluate the reliability of our method, we applied TandemMod to human lung adenocarcinoma cells (A549), colon cancer cells (HCT116), breast cancer cells (MCF7), liver cancer cells (HEPG2), and leukemia cells (K562)68 to detect m6A sites (Fig. 5h–l). The gene discussed is MRPL54; the disease is colonic neoplasm.