To further investigate the role of the interaction between WDR5 and PTENα-NTE SSSRRSS motif in cancer progression, a WDR5 knockout SMMC-7721 cell line (SMMC-7721-WDR5KO) was generated and rescued it with empty vector (EV), wild-type WDR5, or WDR5-2A mutant (F133A/263A) (Supplementary Fig. S8A). Here, WDR5 is linked to cancer.