In the heart failure mouse model and SIRT3 knockout mouse model, 2-APQC inhibited myocardial hypertrophy and relieved myocardial fibrosis by regulating the AKT-mTOR and TGF-β-Smad3 signaling pathways through SIRT3 activation. The gene discussed is SMAD3; the disease is Myocardial fibrosis.