The addition of mTOR/p70S6K pathway inhibitor PI3K/Akt/mTOR IN-2 and TGF-β/Smad3 pathway inhibitor BT173 further proves that 2-APQC improves fibrosis induced HF by blocking the mTOR/p70S6K pathway and TGF-β/Smad3 pathway to inhibit the development of cardiac fibrosis. This evidence concerns the gene SMAD3 and hydrops fetalis.