I1–I5 were sporadic cases diagnosed with developmental and epileptic or epileptic encephalopathy (DEE/EE) that harbored the following heterozygous de novo ANO4 missense variants, respectively: I1, c.1688T>A (p.Met563Lys) (M563K); I2, c.1674C>A (p.Asn558Lys) (N558K); I3, c.1684A>T (p.Ile562Phe) (I562F); I4, c.1807A>G (p.Asn603Asp) (N603D); and I5, c.387C>G (p.Asn129Lys) (N129K). The gene discussed is ANO4; the disease is ethylmalonic encephalopathy.